In a prospective longitudinal cohort study, 40 drug-susceptible pulmonary TB patients (aged 18-69 years 60% male) were enrolled during the first 56 days of the standard 4-drug therapy. Among both LAM and MGIT MTB-culture-positive samples, log 10-transformed LAM concentration and MGIT time to detection (TTD) showed a good inverse relationship (r = −0.803, p < 0.0001). The LAM-ELISA detected all smear- and MTB-culture–positive samples ( n = 70) and 50% ( n = 29) of smear-negative but culture-positive samples ( n = 58) (versus 79.3% 46 positive cases by the Xpert MTB/RIF), but none from non-TB patients ( n = 56). Some sputum specimens were also examined by Xpert MTB/RIF. In a case-control cohort diagnostic study, sputum specimens were collected from 308 patients (aged 17-69 years 62% male) diagnosed as having pulmonary TB diseases or non-TB diseases, but who could expectorate sputum, and were then evaluated by smear microscopy, BACTEC MGIT 960 Mycobacterial Detection System (MGIT) and Lowenstein-Jensen (LJ) culture, and LAM-ELISA. Two clinical studies were performed between the years 20 in Manila, Philippines, in patients without known human immunodeficiency virus (HIV) coinfection. It detected slow-growing NTMs but without cross-reacting to common oral bacteria. The LAM-ELISA had a lower limit of quantification of 15 pg/mL LAM, corresponding to 121 colony-forming units (CFUs)/mL of MTB strain H37Rv.

Using these antibodies, a sandwich enzyme-linked immunosorbent assay (LAM-ELISA) was developed to quantitate LAM concentration. Phage display technology was used to isolate monoclonal antibodies binding to epitopes unique in LAM from MTB and slow-growing nontuberculous mycobacteria (NTM). Masanori Kawasaki, Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Software, Supervision, Validation, Writing – original draft, Writing – review & editing, 1 Carmenchu Echiverri, Data curation, Investigation, Project administration, Resources, Writing – review & editing, 2 Lawrence Raymond, Data curation, Investigation, Methodology, Resources, Writing – review & editing, 3 Elizabeth Cadena, Data curation, Investigation, Methodology, Project administration, Writing – review & editing, 4 Evelyn Reside, Data curation, Investigation, Writing – review & editing, 5 Maria Tarcela Gler, Data curation, Investigation, Methodology, Project administration, Resources, Supervision, Writing – review & editing, 6, ¤ Tetsuya Oda, Data curation, Formal analysis, Writing – review & editing, 1 Ryuta Ito, Data curation, Formal analysis, Writing – review & editing, 1 Ryo Higashiyama, Data curation, Formal analysis, Methodology, Validation, Writing – review & editing, 1 Kiyonori Katsuragi, Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Supervision, Validation, Writing – review & editing, 1 and Yongge Liu, Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Writing – original draft, Writing – review & editing 7, *

Eliza lam tuberculosis